Correction for Substrate Selectivity of Epidermal Growth Factor-Receptor Ligand Sheddases and their Regulation by Phorbol Esters and Calcium Influx

wish to make a correction. The construct TTKK in Figure 4 of the article should be labeled TTTK, since it encodes a chimeric protein with the extracellular domain and transmembrane domain of ADAM17 (TACE, T) fused to the cytoplasmic domain of ADAM10 (KUZ, K). This correction applies to all results obtained with the TTKK construct in Figure 4B, Figure 6D, and Supplemental Figure 2. Therefore, no conclusions about the requirement of the transmembrane domain of ADAM17 for its response to stimulation with PMA can be drawn from these findings. A corrected Figure 4 is included below. The authors sincerely apologize for any consequences this incorrect labeling might have had for studies based on these results. Figure 4. Rescue of constitutive and PMA-stimulated shedding of TGF␣ in Adam17Ϫ/Ϫ cells by chimera between ADAM10 and 17 (A) Diagram of chimeras between ADAM10 and ADAM17 (K, KUZ or kuzbanian, ADAM10; T, TACE, TNF␣-converting enzyme, ADAM17). Domains of ADAM10 and ADAM17 are represented by solid bars and open bars, respectively. An HA-epitope was added to ADAM10, ADAM17, and all the chimeric constructs to facilitate their detection and comparison of transfection levels. A 6xMyc tag and V5-his tag were added to the ⌬-cyto mutants ADAM17b and ADAM10b, respectively. (B) TGF␣ was cotransfected with wild-type or mutant ADAMs in Adam17Ϫ/Ϫ cells as indicated. The cells were incubated with or without PMA (25 ng/ml) for 1 h, and TGF␣ shedding is presented as a ratio of the AP activity in the supernatant and the cell lysate as described in Materials and Methods.